ETHANOL PRODUCER'S DATA BASE

Simplicity in Applied technology

pH in Fermentation

 
  • The "p" in pH is always written as lower case, not upper case.
  • The "p" stands for potential. The "H" stands for Hydrogen.
  • pH of a fermentation will go more acidic as the ferment progresses due to yeast metabolism            byproducts being excreted into the fermenting solution.
  • For this reason, we in the beverage brewing/distilling world start our fermentations at the range of
    • pH 5 - pH 5.5 so that when finished, the pH will still be around pH 4 which ensures we don't stop   or retard the yeast action too soon ( i.e. before all the sugars are converted to ethanol & CO2 ).
  • pH scale is an inverse logarithmic representation of Hydrogen-Ion ( H+ ) concentration in a solution.    
    • Each individual pH unit is a factor of 10 different than the next higher or lower unit.
    • This means that pH 6.0 is 10 times ( 10 x 1 ) more acidic than pH 7.0,
    • and pH 5.0 is 100 times ( 10 x 10 ) more acidic than pH 7.0.
  • Given all of the above, it is clear that the correct pH conditions (slightly acid) of fermentations at start will retard bacterial growth, allowing a correct yeast growth & metabolism environment and ensure virtually trouble-free progress to completion. 
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  • The pH is a measure of the acidity or alkalinity of an aqueous solution expressed on a scale of 1-14.
    • Neutral is pH 7, pH 1-7 is acid, and pH 7-14 is alkaline.

 

  • The pH is measured with an electronic pH meter or test papers that change color according to the pH of the solution being tested.
    • These meters and papers are available from swimming pool supply houses, garden shops, and laboratory supply companies.

 

  • Control of pH during the mashing and fermentation process is important for two reasons:
  • The growth of harmful bacteria is retarded by acid solutions.
    • Yeast will grow only in a mildly acid solution.

  • Ideal pH level during fermentation is absolutely essential. 
  • When pH drops below about 4.2 all fermentation stops.
  • Yeast needs a slightly acid environment in order to grow.
  • So, for best results pH should be kept between 4.8 and 5.0.

 

  • Most grain mashes have a naturally acid pH of between 5.4 and 5.6 after conversion has been accomplished.
  • Other materials, notably saccharine substances like molasses and fruit pressings, have a naturally alkaline pH and must be acidified prior to fermentation.

 

  • The principal bacterial contaminants in a distillery are those that form lactic acid.
  • Although the production of fuel alcohol is not concerned with the taste of the product,
    • Lactic acid formed subtracts from the yield of alcohol.
  • The production of lactic acid and other contaminants should therefore be avoided as much as possible.
  • Below about 4.1 to 4.4 is detrimental to other processes taking place during fermentation.
  • The development of these micro-organisms is severely repressed at pH values under 5.0.
  • Above 5.0 their growth is rapid.
  • The optimum pH range then is 4.8 to 5.0.
  • Consequently, the pH should be checked during the cooking and conversion. 
  • LOWERING pH: If it is above 5.0, it should be reduced by the addition of acid.
  • The acid most commonly used is sulfuric, although any mineral acid is perfectly suitable. 
  • Hydrochloric (muriatic) acid, for example, is available from swimming pool suppliers. 
  • The acid should be added cautiously, the mash stirred, and the pH checked.
  • RAISING pH: To raise the pH, use with sodium hydroxide caustic soda or ordinary lime.
  • CAUTION: Do not over correct, or you may have to scrap the mash.

 

pH Scale

Adjusting pH 

 The pH scale ate left shows the relative acidity of a solution.

It also shows the additives in order to adjust the pH to the desired precise Fermentation levels.

To Lower pH: Add more acid materials such as sulphuric or muriatic acid.

To Raise pH: Add more basic materials such as Lye, Amonia, Baking Soda. 

Supplies

Optimum pH levels for Yeast activity

  • The optimum pH for yeast activity is 4-6.
  • The fact is, that the pH of the cell interior of the yeast remains quite constant at about
  • pH 5.8, regardless of any relatively wide pH variations in the fermenting medium. 
  • The enzymes involved in fermentation thus operate in an optimum pH environment within                           the yeast cell that is largely unaffected by external changes in pH.